Catalase is an enzyme that is found in the cells of many organisms, including plants and animals. It is responsible for catalyzing the breakdown of hydrogen peroxide, a toxic compound that can damage cells, into water and oxygen. This process is important for maintaining the health of cells and preventing oxidative stress, which can lead to cell death.
One way to study the activity of catalase is through an enzyme experiment. In this type of experiment, the rate of catalase activity can be measured by observing the production of oxygen gas as hydrogen peroxide is broken down. This can be done by adding a small amount of catalase to a solution of hydrogen peroxide and then measuring the rate at which oxygen is produced.
There are several variables that can affect the activity of catalase in this type of experiment. One important variable is the concentration of hydrogen peroxide, which can influence the rate of catalase activity. Higher concentrations of hydrogen peroxide can result in faster rates of oxygen production, while lower concentrations may result in slower rates.
Temperature is also an important variable in catalase experiments. Enzymes, including catalase, are sensitive to temperature and their activity can be influenced by changes in temperature. In general, higher temperatures tend to increase the activity of enzymes, while lower temperatures can decrease their activity.
pH is another important variable that can affect the activity of catalase. The pH of the solution in which the enzyme is placed can influence the ionization state of the enzyme, which can in turn affect its activity. In general, enzymes tend to work best at a specific pH, known as their optimal pH, and their activity can decrease outside of this range.
Finally, the presence of inhibitors can also affect the activity of catalase. Inhibitors are substances that can bind to the enzyme and block its activity, either by preventing the substrate from binding to the enzyme or by altering the structure of the enzyme. The presence of inhibitors can significantly decrease the activity of catalase in an experiment.
In conclusion, the activity of catalase can be studied through an enzyme experiment in which the production of oxygen gas is measured as hydrogen peroxide is broken down. There are several variables that can affect the activity of catalase in this type of experiment, including the concentration of hydrogen peroxide, the temperature of the solution, the pH of the solution, and the presence of inhibitors. Understanding the factors that influence the activity of catalase can help researchers to better understand the role of this enzyme in cells and how it contributes to maintaining the health of organisms.
Estimation of Catalase
So if the apparatus is free of leaks, 22 cm 3 of water should be displaced in the measuring cylinder with 10 vol hydrogen peroxide. With a spoon or fork, remove three pieces of potato and place it into a small dish or plastic bag. The aim of this experiment is to see can the enzyme functions normally in different environment pH, temperature and salt concentration via using starch solution, amylase from saliva, 0. The lab will require a catalase, found in potatoes, and hydrogen peroxide. An example of an enzyme catalyzed reaction is enzymatic hydrolysis of an artificial substrate, o-Nitrophenylgalactoside ONPG used in place of lactose. In this activity you will find out how this enzyme works by turning a banana from yellow to brown in just a matter of seconds. Place potatoes into the pot and just cover with cold water.
Carefully add first potato pieces removed into the first test tube or glass of hydrogen peroxide. This does not impact the results of the experiment. Cut them on separate cutting boards and be sure to clean the knife between the two. The oxygen produced in 30 seconds is collected over water. The solutions were stirred and the steps for part two were repeated using different volumes of water and hydrogen peroxide, as shown on the table. In this science activity you will investigate one of these enzymes, called catalase, to find out how it helps to protect your body from cell damage.
Repeat the catalase reaction but this time vary conditions such as the pH by adding vinegar or baking soda, or change the reaction temperature by heating the solution in the microwave. Conical flasks, Pipette, Burette, Measuring cylinder etc. Next, the graduated cylinder, used for measuring the amount of water and extract, was rinsed and 32 ml of distilled water added and stirred with a stirring rod. Use a 100 cm 3 measuring cylinder for concentrations of hydrogen peroxide over 20 vol. Lesson organisation You could run this investigation as a demonstration at two different concentrations, or with groups of students each working with a different concentration of hydrogen peroxide.
Catalase is a very common enzyme that is present in almost all organisms that are exposed to oxygen. Theories like protein structures and intermolecular forces were justified by the results of this lab as well. Catalase, superoxide dismutase, and virulence of Staphylococcus aureus. This blog shares information and resources about pathogenic bacteria, viruses, fungi, and parasites. This can be done easily by putting some of the puree in the fridge and some in the microwave or boil it at home ahead of time. For example when the Catalase was placed in test tubes of 4c and 100c it produced less oxygen or none at all.
Once the reaction is completed, the reaction products are released from the active site of the enzyme. However, at some point you will reach a substrate concentration at which the enzyme gets saturated and becomes the limiting factor. Week 6, the effects of temperature and inhibitor on a reaction rate were monitored. Finally, the average of the three tests per hydrogen peroxide solution were recorded on a chart and the work area was cleaned up. In each of the different measurements of hydrogen peroxide the saturation points are different. Catalase decomposes, or breaks down, hydrogen peroxide into water and oxygen.
How will this affect the results? Enzymes are extremely efficient and may be used over and over again. Ask students to explain how values over 22 cm 3 could happen. PMID: 1117067; PMCID: PMC301784. It was expected that an extreme temperature would decrease the rate of reaction and results observed support that idea. Enzyme Turnip Peroxidase Lab Report 1117 Words 5 Pages The three things that can cause the enzyme to denature is a large change in pH level, High Temperature, and substrate concentration. Raw potatoes and liver will act as the tissue samples. Effect Of Enzyme Concentration On Enzyme Reaction 1046 Words 5 Pages ABSTRACT: The purpose of the experiments for week 5 and week 6 support each other in the further understanding of enzyme reactions.
Overall, how would you describe the relationship between pH and Catalase activity? Be sure to wash any utensils that touch another sample before using them again. The bubbling that you may see if you pour hydrogen peroxide on a cut is the oxygen gas produced from catalase activity. The goal of this experiment is to measure the catalase activity. Bio Chem: Lab Report Of Amylase In Enzyme 916 Words 4 Pages Bio Chem lab Report 04 Enzyme Biochemistry Group Member: Chan Man Jeun Duncan 16002621 Law Sze Man 16000478 Introduction Enzyme is a protein base structure substance in our body. Health and safety checked, September 2008 Downloads Download the student sheet Web links Website accessed October 2011.
Effect Of Temperature On Amylase 1167 Words 5 Pages These enzymes have a secondary and tertiary structure and this could be affected by increases and decreases in temperature beyond the optimum temperature of the enzyme to work in. Enzymes have the ability to lower the activation energy of a chemical reaction by interacting with its reactants. Place a beaker of peroxide in an ice bath, and another in a warm water bath. A catalyst gets reactions started and makes them happen faster by increasing the rate of a reaction that otherwise might not happen at all, or would take too long to sustain life. Similarly their nature as large protein molecules whose catalytic activity can be very specific to certain chemical reactions may be unfamiliar. If there is no hydrogen peroxide present, the catalase cannot function, which is why in cup 1 you should not have seen any bubble or foam production.